List of Receptors

Receptor Organism Cell type Mechanism of raft association Methods used to detect raft association Functional implication
Adenosine A1 receptor

Rat 1, Pig 2, Syrian hamster 3

Ventricular cardiomyocyte 1; Kidney epithelial cell 2 Smooth muscle cell line 3

Bound caveolin-3  1 and caveolin-13

Detergent-free 1, 2 Electron microscopy 3; Confocal microscopy 3

Agonist stimulation leads to translocation out of 1or into caveolae and caveolae-dependent internalization 2,3
Adenosine A2A receptor

Dog 4; Rat 5

CHO cells 4, Neonatal spinal cord 5

Interact with caveolin-14

Confocal microscopy 4; Detergent-dependent 5

Internalization via raft-dependent mechanism 4
Adenosine A2B receptor

Human 6

Intestinal epithelial cell line 6

n.a

Detergent-free 6

The receptor localization in caveolae was increased after apical or basolateral stimulation of receptor with adenosine 6
Angiotensin II type 1 receptor

Rat 7, 8; Human 9

VSMC 7, 10, 11; BHK and HEK cells 8; COS-7 cells 9

Bound caveolin-17

Detergent-free7,8; Detergent-dependent 8; Electron microscopy 9

Agonist stimulation leads to translocation to caveolae 7 or non-caveolin lipid raft 8 or has no effect on localization 9; Caveolin-binding mutation reduces the plasma membrane expression, agonist-dependent internalization and phospholipase C production 9; Microtubules are essential in translocating the AT1 receptor to caveolae 10; Receptors movement into caveolae is necessary for ROS production, Rac1 activation, Sos-1 tyrosine phosphorylation, ROS-dependent Akt phosphorylation and VSMC hypertrophy 11
Bradykinin B1 receptor

Rabbit 12

HEK-293 12, 13

Putative caveolin binding motif 13

Confocal microscopy 12; Immunoblotting 13

Agonist induced caveolae translocation to caveolae12; Receptor localization in CLR did not changed in response to agonist 13
Bradykinin B2 receptor

Hamster 14; Human 13

HEK 293 13; DDT1-MF-2 smooth muscle cell 14; Epithelial carcinoma cell line 15; Aortic endothelial cells 16

Putative caveolin binding motif 13

Detergent-free 14, 16; Detergent-dependent 14, Confocal microscopy 15, Immunoblotting 13

Agonist BK induced movement of receptor, Gαq and Gαi to caveolae 15, 14; BK treatment leads to translocation of receptor out of caveolae 16; OR had no effect on localization 13
Calcium-sensing receptor

Bovine 17; Human 18

Parathyroid cells 17; Saos-2 cells 18; Cardiomyocytes 19

Bound caveolin-117 AND caveolin-3 19

Detergent-free 17,19, 18; Detergent-dependent 18

Ischemic preconditioning increases ERK1/2 phosphorylation in caveolin-3-enriched fraction 19
Cannabinoid receptor

Human 20, 21

Breast cancer MDA-MB-231 cells20; HEK-293 22,21

n.a

Detergent-free based on Opti prep density gradient 20; Confocal microscopy 20

Agonist treamtnet decreases the receptor associated with lipid raft 20; Receptor partially internalized via caveolae 22
CC chemokine receptor type 5 (CCR5)

Human 23, 24, 25

Human breast carcinoma MCF-7 cells 26; HeLa cells24; GEM NKR T cells 23; CHO cells25

Palmitoylation of C-terminal domain 27

Detergent-dependent 23; Immunofluorescence microscopy 24

Functional receptor localizes to lipid raft upon agonist treatment 23; CCR5 internalized via caveolae-dependent way 25
CC chemokine receptor type 6 (CCR6)

Human 28

Jurkat cells 28

n.a

Detergent-dependent 28

MβCD treatment impaired receptor-induced chemotaxis 28