Bradykinin B2 receptor

Organism	Hamster 1; Human 2 1. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7.
Functional implication	Agonist BK induced movement of receptor, Gαq and Gαi to caveolae 3, 1; BK treatment leads to translocation of receptor out of caveolae 4; OR had no effect on localization 2 1. a. b. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Methods used to detect raft association	Detergent-free 1, 4; Detergent-dependent 1, Confocal microscopy 3, Immunoblotting 2 1. a. b. c. d. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Mechanism of raft association	Putative caveolin binding motif 2 1. a. b. c. d. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. d. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Caveolin-binding motif	Yes 2 1. a. b. c. d. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. d. e. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Cell type	HEK 293 2; DDT1-MF-2 smooth muscle cell 1; Epithelial carcinoma cell line 3; Aortic endothelial cells 4 1. a. b. c. d. e. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. d. e. f. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. c. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. c. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Species	Human 2, 3; Hamster 1; Bovine 4 1. a. b. c. d. e. f. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. d. e. f. g. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. c. d. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. c. d. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.
Receptor expression	Transient transfection 2; Stable transfection 3; Endogenous 1 1. a. b. c. d. e. f. g. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66. 2. a. b. c. d. e. f. g. h. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7. 3. a. b. c. d. e. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28. 4. a. b. c. d. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.

Refrences

Human 2, 3; Hamster 1; Bovine 4

1. a. b. c. d. e. f. [494] de Weerd, WF, Leeb-Lundberg LM. 1997. Bradykinin sequesters B2 bradykinin receptors and the receptor-coupled Galpha subunits Galphaq and Galphai in caveolae in DDT1 MF-2 smooth muscle cells.. The Journal of biological chemistry. 272(28):17858-66.
2. a. b. c. d. e. f. g. [385] Lamb, ME, Zhang C, Shea T, Kyle DJ, Leeb-Lundberg LM. 2002. Human B1 and B2 bradykinin receptors and their agonists target caveolae-related lipid rafts to different degrees in HEK293 cells. Biochemistry. 41:14340-7.
3. a. b. c. d. [368] Haasemann, M, Cartaud J, Muller-Esterl W, Dunia I. 1998. Agonist-induced redistribution of bradykinin B2 receptor in caveolae. J Cell Sci. 111 ( Pt 7):917-28.
4. a. b. c. d. [375] Ju, H, Venema VJ, Liang H, Harris MB, Zou R, Venema RC. 2000. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J. 351:257-64.

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Bradykinin B2 receptor