List of Receptors

Receptor Organism Cell type Mechanism of raft association Methods used to detect raft association Functional implication
Glucagon-like peptide 2 receptor (GLP-2)

Human 1

DLD-1 and BHK cells 1

n.a

Detergent-dependent 1; Western blot 1

MβCD treatment inhibited receptor internalization and desensitization but not adenyly cyclase acivity 1
Histamin H1 receptor

Human 2

CHO-K1 cells 2

n.a

Confocal microscopy 2

Filipin or nystatin prevented histamine-induced internalization of receptor 2
Leukotriene B receptor

Human 3; Rat 4

Neutrophil 3; Alveolar macrophage 4

n.a

Detergent-dependent 3; Fluorescence resonance energy transfer (FRET) 3; Detergent-free 4

Agonist treatment did not alter receptor localization 3; MβCD treatment abolished LTB4-induced ca2+ mobilization and ERK1/2 phosphorylation 3; After igG-RBC treamtment receptor moved to lipid raft 4 
Lipoxin A4 receptor

Human 5

HEK-293 5

n.a

Detergent-dependent 5

MβCD treatment reduces LXA4-dependent Platelet-derived growth factor receptor β dephosphorylation 5; LXA4 binding to receptor promotes SHP-2 recruitment to lipid raft 5
Luteinizing hormone receptor

Rat 6

CHO cells 6

n.a

Detergent-dependent 6

After human chorionic gonadotropin binding, receptors move to lipid raft 6,7; MβCD treatment reduced signaling 6
Lysophosphatidic acid receptor

Pig 8

pCMVECs 8; COS-7 cells 9

n.a

Immunoprecipitation 8; Western blot 8

MβCD treatment reduces LPA-inducd ERK1/2 phosphorylation 8
M2 muscarinic receptor

Rat 10, 11

Ventricular myocytes 10; Cardiac myocytes 11; COS-7 cells 11

n.a

Detergent-free 10,11; Immunoprecipitation 10

Receptor translocates in to caveolae upon agonist treatment 10, 11; Possibly internalization by raft –dependent mechanism which negatively controls NO signaling 11
M3 muscarinic receptor

Human 12, 13, 14, Canine 12

Airway smooth muscle cells 12, 13; Detrusor muscle  15

n.a

Disruption of caveolae12, 13; Immunoelectron microscopy 14

Caveolae disruption by cyclodextrin and caveolin-1 inhibition impairs ca2+ regulation12, 13; M3 muscarinic receptor colocalized with KATP channel subunit Kir6.1  in caveolae 14.
Mammalian gonadotropin releasing hormone receptor (mGnRHR)

Mouse 16

Gonadotrope-derived α-T3-1 cell, CHO 16

n.a

Detergent-free 16, Detergent-dependent 16

Cyclodextrin treatment attenuates GnRHR activation of ERK and induction of c-fos gene expression16
Melanin-concentrating hormone receptor (MCHR)

-

CHO-K1 cells 17

Bound caveolin-1 17

Detergent-free 17

MβCD treatment impaired MCH-mediated ERK signaling 17; There was no significant change in receptor localization after MCH treatment 17