List of Receptors

Receptor Organism Cell type Mechanism of raft association Methods used to detect raft association Functional implication
Protease-activated receptor type 1 (PAR-1)

Bovine 1; Human 2, 3

Aortic endothelial cells 1; HEK-293 2, COS-7 cells 2; Endothelial cells 3

Bound caveolin-1 2

Detergent-free 1, 2; Immunoaffinity isolation 1; Immunoprecipitation 2; Confocal microscopy 2

Filipin treatment attenuates Myosine light chain phosphorylation and stress fiber formation 1; Agonist treatment did not change receptor localization in raft 2; PAR-1 signaling by APC is lost in caveolin-1 deficient cells 3; Lipid raft/caveolae is essential for N-terminal truncation of PAR1 which is needed for its activation and PAR1-mediated signaling by APC 3
Protease-activated receptor type 2 PAR-2

Mouse 4; Human 5

Epidermis 4; Breast carcinoma cells line MDA-MB-231 5

n.a

Western immunoblot 4; Detergent-dependent 5; Immunofluorescence confocal microscopy 5

Receptor activation and barrier abrogation leads to receptor internalization 4;  MβCD  and filipin treatment in addition to caveolin-1 silencing reduced TF-VIIa-mediated cleavage of PAR-2 and IL-8 gene expression 5
Serotonin 5-HT 7 receptor

Human 6

HeLa cells 6

n.a

Sphingomyelin depletion

Fumonisin B1 and PDMP treatment decrease remarkably binding of 5-HT to 5-HT7 receptor 6
Serotonin 5-HT1A receptor

Mouse 7

NIH-3T3 cells 7; CHO cells 7

Palmitoylation 7

Gradient centrifugation 7, Copatching assay 7

Signaling reduces in cyclodextrin treated and palmitoylated-deficient cells 7
Serotonin 5-HT2A receptor

Rat 8

Tail artery 8

n.a

Detergent-free 8

Cyclodextrin reduce agonist response 8
Somatostatin receptor type 2

Human 9

Glioma cells 9

n.a

Electron microscopy 9; Biochemical technique 9

Receptor internalized via caveolin-positive endosomal vesicles after ligand binding 9
Sphingosine 1-phosphate receptor

Human 10; Murine 11

COS-7 cells 10; CHO cells 11

Bound caveolin 10; N-glycan 11

Detergent-free 10

Agonist induced translocation to the caveolae, Caveolin overexpression inhibited receptor function 10
Thyrotropin receptor

Human 12, 13

CHO cells 12,13; Thyrocytes 13

n.a

Confocal microscopy 12; Detergent-free 13

Receptors move out of raft upon activation 12; MβCD treatment did not alter cAMP accumulation 13; Cav-1 knockdown increased cAMP accumulation 14
Thyrotropin-releasing hormone receptor

Rat 15, 16

HEK-293 15, 16, 17

n.a

Sucrose gradient centrifugation and immunoblotting 15

Agonist induced receptor movement in to low density vesicular fraction 15; MβCD treatment inhibited Gq/G11α tyrosine phosphorylation 16
Vasopressin receptor

-

Endothelium of tail artery 8; Thick ascending limb of the loop of Henle 18

n.a

Detergent-free 8; Detergent-dependent 18

MβCD treatment reduced receptor function8; Lipid raft is required for receptor-induced trafficking 18