Thyrotropin-releasing hormone receptor

Organism	Rat 1, 2 1. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32.
Functional implication	Agonist induced receptor movement in to low density vesicular fraction 1; MβCD treatment inhibited Gq/G11α tyrosine phosphorylation 2 1. a. b. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32.
Methods used to detect raft association	Sucrose gradient centrifugation and immunoblotting 1 1. a. b. c. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32.
Mechanism of raft association	n.a 1. a. b. c. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32.
Caveolin-binding motif
Cell type	HEK-293 1, 2, 3 1. a. b. c. d. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. c. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32. 3. [416] Rudajev, V, Novotny J, Hejnova L, Milligan G, Svoboda P. 2005. Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein. J Biochem. 138:111-25.
Species	Human 1, 2, 3 1. a. b. c. d. e. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. c. d. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32. 3. a. b. [416] Rudajev, V, Novotny J, Hejnova L, Milligan G, Svoboda P. 2005. Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein. J Biochem. 138:111-25.
Receptor expression	Stable transfection 1, 2, 3 1. a. b. c. d. e. f. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38. 2. a. b. c. d. e. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32. 3. a. b. c. [416] Rudajev, V, Novotny J, Hejnova L, Milligan G, Svoboda P. 2005. Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein. J Biochem. 138:111-25.

Refrences

Human 1, 2, 3

1. a. b. c. d. e. [356] Drmota, T, Novotny J, Gould GW, Svoboda P, Milligan G. 1999. Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation. Biochem J. 340 ( Pt 2):529-38.
2. a. b. c. d. [396] Matousek, P, Durchankova D, Svandova I, Novotny J, Svoboda P. 2005. Agonist-induced tyrosine phosphorylation of Gq/G11 alpha requires the intact structure of membrane domains. Biochem Biophys Res Commun. 328:526-32.
3. a. b. [416] Rudajev, V, Novotny J, Hejnova L, Milligan G, Svoboda P. 2005. Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein. J Biochem. 138:111-25.

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Thyrotropin-releasing hormone receptor