Organism |
Bovine ; Human ,
|
Functional implication |
Filipin treatment attenuates Myosine light chain phosphorylation and stress fiber formation ; Agonist treatment did not change receptor localization in raft ; PAR-1 signaling by APC is lost in caveolin-1 deficient cells ; Lipid raft/caveolae is essential for N-terminal truncation of PAR1 which is needed for its activation and PAR1-mediated signaling by APC
|
Methods used to detect raft association |
Detergent-free , ; Immunoaffinity isolation ; Immunoprecipitation ; Confocal microscopy
|
Mechanism of raft association |
Bound caveolin-1
|
Caveolin-binding motif |
|
Cell type |
Aortic endothelial cells ; HEK-293 , COS-7 cells ; Endothelial cells
|
Species |
Bovine ; Human , ; Monkey
|
Receptor expression |
Endogenous ; HEK-293: stable transfection ; COS-7: transient transfection
|
Bovine ; Human , ; Monkey