Protease-activated receptor type 1 (PAR-1)

Organism

Bovine 1; Human 2, 3

Functional implication

Filipin treatment attenuates Myosine light chain phosphorylation and stress fiber formation 1; Agonist treatment did not change receptor localization in raft 2; PAR-1 signaling by APC is lost in caveolin-1 deficient cells 3; Lipid raft/caveolae is essential for N-terminal truncation of PAR1 which is needed for its activation and PAR1-mediated signaling by APC 3

Methods used to detect raft association

Detergent-free 1, 2; Immunoaffinity isolation 1; Immunoprecipitation 2; Confocal microscopy 2

Mechanism of raft association

Bound caveolin-1 2

Caveolin-binding motif
Cell type

Aortic endothelial cells 1; HEK-293 2, COS-7 cells 2; Endothelial cells 3

Species

Bovine 1; Human  2, 3; Monkey 2

Receptor expression

Endogenous 1; HEK-293: stable transfection 2; COS-7: transient transfection 2

Refrences