CC chemokine receptor type 5 (CCR5)

Organism	Human 1, 2, 3 1. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91.
Functional implication	Functional receptor localizes to lipid raft upon agonist treatment 1; CCR5 internalized via caveolae-dependent way 3 1. a. b. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91.
Methods used to detect raft association	Detergent-dependent 1; Immunofluorescence microscopy 2 1. a. b. c. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. a. b. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91.
Mechanism of raft association	Palmitoylation of C-terminal domain 4 1. a. b. c. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. a. b. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91. 4. [435] Venkatesan, S, Rose JJ, Lodge R, Murphy PM, Foley JF. 2003. Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4. Mol Biol Cell. 14:3305-24.
Caveolin-binding motif
Cell type	Human breast carcinoma MCF-7 cells 5; HeLa cells2; GEM NKR T cells 1; CHO cells3 1. a. b. c. d. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. a. b. c. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. c. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91. 4. [435] Venkatesan, S, Rose JJ, Lodge R, Murphy PM, Foley JF. 2003. Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4. Mol Biol Cell. 14:3305-24. 5. [395] Manes, S, Mira E, Gomez-Mouton C, Lacalle RA, Keller P, Labrador JP, Martinez AC. 1999. Membrane raft microdomains mediate front-rear polarity in migrating cells. EMBO J. 18:6211-20.
Species	Human 1; 2, 5; Hamster 3 1. a. b. c. d. e. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. a. b. c. d. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. c. d. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91. 4. [435] Venkatesan, S, Rose JJ, Lodge R, Murphy PM, Foley JF. 2003. Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4. Mol Biol Cell. 14:3305-24. 5. a. b. [395] Manes, S, Mira E, Gomez-Mouton C, Lacalle RA, Keller P, Labrador JP, Martinez AC. 1999. Membrane raft microdomains mediate front-rear polarity in migrating cells. EMBO J. 18:6211-20.
Receptor expression	Transient transfection 2; Stable transfection 1, 3 1. a. b. c. d. e. f. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306. 2. a. b. c. d. e. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44. 3. a. b. c. d. e. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91. 4. [435] Venkatesan, S, Rose JJ, Lodge R, Murphy PM, Foley JF. 2003. Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4. Mol Biol Cell. 14:3305-24. 5. a. b. [395] Manes, S, Mira E, Gomez-Mouton C, Lacalle RA, Keller P, Labrador JP, Martinez AC. 1999. Membrane raft microdomains mediate front-rear polarity in migrating cells. EMBO J. 18:6211-20.

Refrences

Human 1; 2, 5; Hamster 3

1. a. b. c. d. e. [402] Nguyen, DH, Taub D. 2002. Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5. Blood. 99:4298-306.
2. a. b. c. d. [408] Percherancier, Y, Planchenault T, Valenzuela-Fernandez A, Virelizier JL, Arenzana-Seisdedos F, Bachelerie F. 2001. Palmitoylation-dependent control of degradation, life span, and membrane expression of the CCR5 receptor. J Biol Chem. 276:31936-44.
3. a. b. c. d. [400] Mueller, A, Kelly E, Strange PG. 2002. Pathways for internalization and recycling of the chemokine receptor CCR5. Blood. 99:785-91.
4. [435] Venkatesan, S, Rose JJ, Lodge R, Murphy PM, Foley JF. 2003. Distinct mechanisms of agonist-induced endocytosis for human chemokine receptors CCR5 and CXCR4. Mol Biol Cell. 14:3305-24.
5. a. b. [395] Manes, S, Mira E, Gomez-Mouton C, Lacalle RA, Keller P, Labrador JP, Martinez AC. 1999. Membrane raft microdomains mediate front-rear polarity in migrating cells. EMBO J. 18:6211-20.

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CC chemokine receptor type 5 (CCR5)