CXC chemokine receptor type 4 (CXCR4)

Organism	Human 1 1. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6.
Functional implication	Gp120 recruits receptor to lipid raft 2; MβCD treatment inhibits receptor internalization, SDF-1-induced migration and HIV infection 3, 4, 5 1. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.
Methods used to detect raft association	Confocal microscopy 1, 2, 3, 4; Electron microscopy 2; Detergent-dependent 3 1. a. b. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. a. b. c. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. a. b. c. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. a. b. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.
Mechanism of raft association	n.a 1. a. b. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. a. b. c. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. a. b. c. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. a. b. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.
Caveolin-binding motif
Cell type	HEK-293 1; PMBC 1; Sup T1 lymphoblastoid 2; PM1 T cells 3; Hematopoietic progenitor cell line KG1a 4 1. a. b. c. d. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. a. b. c. d. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. a. b. c. d. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. a. b. c. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.
Species	Human 1, 2, 3, 4 1. a. b. c. d. e. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. a. b. c. d. e. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. a. b. c. d. e. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. a. b. c. d. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.
Receptor expression	Endogenous 1; Stable expression 4 1. a. b. c. d. e. f. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6. 2. a. b. c. d. e. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60. 3. a. b. c. d. e. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22. 4. a. b. c. d. e. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10. 5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.

Refrences

Human 1, 2, 3, 4

1. a. b. c. d. e. [394] Manes, S, Del Real G, Lacalle RA, Lucas P, Gomez-Mouton C, Sanchez-Palomino S, Delgado R, Alcami J, Mira E, Martinez AC. 2000. Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1:190-6.
2. a. b. c. d. e. [430] Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A. 2001. Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells. FEBS Lett. 506:55-60.
3. a. b. c. d. e. [410] Popik, W, Alce TM, Au WC. 2002. Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells. J Virol. 76:4709-22.
4. a. b. c. d. [434] van Buul, JD, Voermans C, van Gelderen J, Anthony EC, van der Schoot CE, Hordijk PL. 2003. Leukocyte-endothelium interaction promotes SDF-1-dependent polarization of CXCR4. J Biol Chem. 278:30302-10.
5. [403] Nguyen, DH, Taub D. 2002. CXCR4 function requires membrane cholesterol: implications for HIV infection. J Immunol. 168:4121-6.

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CXC chemokine receptor type 4 (CXCR4)