Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells
| Title | Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells |
| Publication Type | Journal Article |
| Year of Publication | 2001 |
| Authors | Sorice, M, Garofalo T, Misasi R, Longo A, Mattei V, Sale P, Dolo V, Gradini R, Pavan A |
| Journal | FEBS Lett |
| Volume | 506 |
| Pagination | 55-60 |
| Date Published | Sep 28 |
| ISBN Number | 0014-5793 (Print)0014-5793 (Linking) |
| Accession Number | 11591370 |
| Keywords | Cell Line, Cell Membrane/metabolism, Chromatography, Thin Layer, G(M3) Ganglioside/*metabolism, HIV Envelope Protein gp120/metabolism, Humans, Precipitin Tests, Protein Binding, Receptors, CXCR4/*metabolism |
| Abstract | CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within GM3-enriched plasma membrane domains of SupT1 cells and coimmunoprecipitation experiments revealed that CXCR4 was immunoprecipitated by IgG anti-GM3 after gp120 pretreatment. These findings reveal that gp120 binding induces a strict association between CXCR4 and ganglioside GM3, supporting the view that GM3 and CXCR4 are components of a functional multimolecular complex critical for HIV-1 entry. |
| URL | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11591370 |