Multiple mechanisms of CB1 cannabinoid receptors regulation
| Title | Multiple mechanisms of CB1 cannabinoid receptors regulation |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Keren, O, Sarne Y |
| Journal | Brain Res |
| Volume | 980 |
| Pagination | 197-205 |
| Date Published | Aug 8 |
| ISBN Number | 0006-8993 (Print)0006-8993 (Linking) |
| Accession Number | 12867259 |
| Keywords | Animals, Cannabinoids/*metabolism/pharmacology, Cell Line, Down-Regulation/drug effects/*physiology, Endocytosis/drug effects/physiology, Humans, Mice, Protein Binding/drug effects/physiology, Receptors, Cannabinoid, Receptors, Drug/agonists/*metabolism, Tumor Cells, Cultured |
| Abstract | Agonist-induced regulation of cannabinoid CB1 receptors was examined in HEK-293 cells transfected with CB1 receptors and in neuroblastoma N18TG2 cells that naturally express CB1 receptors. In HEK-293 cells, CB1 receptors internalization proceeded, in parallel, via clathrin-coated pits and caveolae. Simultaneous disruption of both pathways induced compensatory endocytic mechanism(s). In N18TG2 cells, endocytosis was not mediated by caveolae-like membrane domains. Heterologous, opioid-induced, downregulation of CB1 receptors was evident in HEK-293 but not N18TG2 cells. The data demonstrate the existence of multiple pathways of CB1 receptors regulation. |
| URL | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12867259 |