Endothelin A receptor

Organism	Rat 1; Human 2 1. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Functional implication	Receptor localization in caveolae is important in signal transduction 1; Agonist induced receptor internalization 2 1. a. b. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Methods used to detect raft association	Coimmunoprecipitation 1 1. a. b. c. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Mechanism of raft association	Bound caveolin 1 1. a. b. c. d. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Caveolin-binding motif
Cell type	COS cells 1 1. a. b. c. d. e. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Species	Hamster 2 1. a. b. c. d. e. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. c. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.
Receptor expression	Tansient transfection 1; Stable transfection 2 1. a. b. c. d. e. f. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32. 2. a. b. c. d. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.

Refrences

Hamster 2

1. a. b. c. d. e. [350] Chun, M, Liyanage UK, Lisanti MP, Lodish HF. 1994. Signal transduction of a G protein-coupled receptor in caveolae: colocalization of endothelin and its receptor with caveolin. Proc Natl Acad Sci U S A. 91:11728-32.
2. a. b. c. [404] Okamoto, Y, Ninomiya H, Miwa S, Masaki T. 2000. Cholesterol oxidation switches the internalization pathway of endothelin receptor type A from caveolae to clathrin-coated pits in Chinese hamster ovary cells. J Biol Chem. 275:6439-46.

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Endothelin A receptor