β2-adrenoreceptor

Organism	Rat 1; Mouse 2 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6.
Functional implication	Agonist treatment leads to movement out of caveolae 3; Caveolae disruption by filipin or cyclodextrin increased cAMP accumulation and contraction rate 2' 3; The receptor colocalized with Adenylyl scyclase 6 (AC6) in lipid raft fractions 4. 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95.
Methods used to detect raft association	Detergent-free 3, 5, 4; Immunoblot analysis 4 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72.
Mechanism of raft association	Bound caveolin-15 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. a. b. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72.
Caveolin-binding motif	Yes 6 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. a. b. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72. 6. [405] Ostrom, RS, Post SR, Insel PA. 2000. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving G(s). J Pharmacol Exp Ther. 294:407-12.
Cell type	COS-7 cells 5, Cardiomyocytes 3; Cardiac fibroblastas 3; Neonatal myocytes 2; bronchial smooth muscle cells 4; HEK-293 4 1. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. c. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. d. e. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. d. e. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. a. b. c. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72. 6. [405] Ostrom, RS, Post SR, Insel PA. 2000. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving G(s). J Pharmacol Exp Ther. 294:407-12.
Species	Monkey 5; Rat 3, 1; Mouse 2, 4; Human 4 1. a. b. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. c. d. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. d. e. f. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. d. e. f. g. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. a. b. c. d. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72. 6. [405] Ostrom, RS, Post SR, Insel PA. 2000. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving G(s). J Pharmacol Exp Ther. 294:407-12.
Receptor expression	Endogenous 1, 2; Transient transfection 5 1. a. b. c. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48. 2. a. b. c. d. e. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6. 3. a. b. c. d. e. f. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57. 4. a. b. c. d. e. f. g. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95. 5. a. b. c. d. e. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72. 6. [405] Ostrom, RS, Post SR, Insel PA. 2000. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving G(s). J Pharmacol Exp Ther. 294:407-12.

Refrences

Monkey 5; Rat 3, 1; Mouse 2, 4; Human 4

1. a. b. [418] Rybin, VO, Pak E, Alcott S, Steinberg SF. 2003. Developmental changes in beta2-adrenergic receptor signaling in ventricular myocytes: the role of Gi proteins and caveolae microdomains. Mol Pharmacol. 63:1338-48.
2. a. b. c. d. [440] Xiang, Y, Rybin VO, Steinberg SF, Kobilka B. 2002. Caveolar localization dictates physiologic signaling of beta 2-adrenoceptors in neonatal cardiac myocytes. J Biol Chem. 277:34280-6.
3. a. b. c. d. e. f. [419] Rybin, VO, Xu X, Lisanti MP, Steinberg SF. 2000. Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway. J Biol Chem. 275:41447-57.
4. a. b. c. d. e. f. g. [502] Bogard, AS, Adris P, Ostrom RS. 2012. Adenylyl cyclase 2 selectively couples to E prostanoid type 2 receptors, whereas adenylyl cyclase 3 is not receptor-regulated in airway smooth muscle.. The Journal of pharmacology and experimental therapeutics. 342(2):586-95.
5. a. b. c. d. [424] Schwencke, C, Okumura S, Yamamoto M, Geng YJ, Ishikawa Y. 1999. Colocalization of beta-adrenergic receptors and caveolin within the plasma membrane. J Cell Biochem. 75:64-72.
6. [405] Ostrom, RS, Post SR, Insel PA. 2000. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving G(s). J Pharmacol Exp Ther. 294:407-12.

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β2-adrenoreceptor